Businesses for Sale in Jacksonville, Florida

Beauty & Personal Care

Picture yourself walking into your own fully equipped medical spa, ready to welcome your first clients tomorrow. This turnkey opportunity in Jacksonville's desirable Mandarin area could be exactly what you've been looking for. You'll love that everything is already done for you. The previous owner invested over $250,000 creating this beautiful, modern space with state-of-the-art equipment, elegant furniture, premium décor, and initial inventory. No waiting months for buildouts or dealing with contractors - you can literally turn the key and start serving clients. Whether you're a medical professional looking to expand, an esthetician ready to be your own boss, or an entrepreneur excited about the booming wellness industry, this spa offers you a solid foundation. The aesthetics and wellness market continues growing rapidly, and you'll be positioned perfectly to capture that demand. The Mandarin location gives you everything you need for success - excellent accessibility for clients, strong local demographics, and room for growth. You won't have to worry about whether people can find you or afford your services. What makes this opportunity special is that you're buying a complete business solution, not just equipment. Every detail has been thoughtfully planned to create that premium experience clients expect from a medical spa. You can focus on what you do best - providing excellent treatments and building relationships with clients - instead of worrying about setup details. This is your chance to step into a turnkey operation where someone else has already solved the hard problems of location, design, and equipment selection. You get to enjoy the exciting part - growing your business and serving clients in a space designed for success. Ready to see yourself as a medical spa owner?

Retail

This gas station and convenience store is ideally situated at the busy intersection of Ramona Blvd and Lane Avenue (SR-103), just south of I-10 at Exit 357. The site boasts excellent visibility from the freeway, with high traffic counts of over 24,000 vehicles daily at the intersection and more than 100,000 along I-10. Spanning approximately 0.755 acres, the property includes a 1,080 sq. ft. convenience store, a separate 608 sq. ft. car wash building, and a 1,240 sq. ft. fuel canopy, featuring 4 Gilbarco Encore 700 MPDs. Zoned CCG-2, it allows for various commercial uses such as service stations, car washes, and retail. The absence of a Fuel Supply Agreement offers new ownership the flexibility to negotiate their own supply and branding. This prime location presents a fantastic opportunity for an experienced owner/operator to enhance and maximize the property.

Health Care & Fitness

Located in the heart of Jacksonville's Southside, this family medical practice has been servicing patients for over 18 years in same location. The ARPN is the owner of the practice, and she employs 2 primary care physicians along with a Chiropractor and 9 other support staff. Family medical services are provided along with physical therapy and cash services such as hormone replacement, peptide, and stem cell treatments. Fully ACHA certified. What a NP can do in Florida: What a Nurse Practitioner is A licensed, independent healthcare provider in all U.S. states. An RN with graduate‑level education (MSN, DNP) and national board certification. Trained to deliver primary, acute, and specialty care across many settings (clinics, hospitals, urgent care, private practice). NPs may practice fully independently, with reduced collaboration, or under physician supervision, depending on state law. Florida currently allows “restricted practice,” meaning NPs must have a collaborative agreement with a physician for certain functions. In Florida an NP can: Within primary care only, autonomous NPs may: Diagnose and treat illnesses Prescribe medications (including controlled substances, Schedules II–V) Order and interpret labs and imaging Admit and discharge patients in certain facilities Sign documents normally reserved for physicians (including death certificates) CURRENTLY THERE ARE TWO ATTENDING PHYSICIAN MD'S.

Entertainment & Recreation

Unique opportunity to own a youth sports recreational football league ! The organization offers competitive football including 7v7, touch, and age-based flag football. Seasonal sports include spring 8v8 tackle and fall 11v11 tackle. In addition the organization provides squad and sideline opportunities like cheerleading. The organization also maintains some corporate partnerships like Under Armour to deliver Under the Lights Football and offers player financial assistance in the form of scholarships. To complete the service offering, the organization supplies needed equipment and runs a food truck during games times for an added revenue stream. Included in the sale is all player equipment, 2 cargo trailers, and 1 completely equipment food truck.

Health Care & Fitness

This listing features a veterinary practice located in the Jacksonville area, offering a unique opportunity in the health care sector. Interested parties will need to acknowledge a Confidentiality and Non-Disclosure Agreement.

Transportation & Storage

Established in 1998, this luxury transportation service has been a staple in North Florida, offering premium chauffeured solutions for weddings, proms, corporate events, and more. The business operates from home and boasts an SEO-optimized website that ranks highly in regional searches, complemented by effective Google Ad campaigns. With a focus on maintaining an excellent work-life balance, the company operates under capacity to allow for growth. The sale includes a fleet of four vehicles, proprietary booking software, and a robust customer database. The retiring owner, with 26 years of experience, will assist with a two-week onboarding process.

Restaurants & Food

A long time established meat and seafood grocery store that has been in the business and in the same location for over fifty years. In addition to meat and seafood retail the business sells a limited vegetables and fruit items as well as non perishable canned foods. The current owner expanded the business offering fried chicken.

Building & Construction

This family-run flooring company has earned a stellar reputation over more than 50 years. They specialize in a wide range of products including luxury vinyl plank, porcelain tile, engineered hardwood, window treatments, commercial flooring, and various carpeting options, all aimed at assisting families in making informed choices that fit their homes and budgets.

Real Estate

Rare chance to own 187.89 acres of waterfront industrial land in Jacksonville, Florida, ideal for a large-scale AI data center and energy infrastructure campus. This site offers access to essential utilities and transportation links, presenting an opportunity for a comprehensive data center and energy development strategy.

Service Businesses

Established full-service dry cleaning and laundry business featuring a fully equipped production facility with substantial capacity for additional volume. The operation includes professional dry-cleaning equipment, shirt finishing equipment, pressing stations, conveyors, laundry equipment, compressors, and other production assets. The current owners have intentionally reduced their involvement as they transition into retirement, creating an opportunity for a new hands-on owner to expand operations. The business has a long-standing reputation and an established customer base. Seller is willing to provide an extended transition and training period to support a successful ownership transfer.

Wholesale & Distributors

Established and profitable, this industrial supply company has built a strong reputation for providing quality products, dependable service, and exceptional customer support. The business serves a diverse commercial and industrial customer base by offering a broad range of industrial products and related solutions designed to support efficient day-to-day operations. A long history of reliability and responsiveness has resulted in strong customer loyalty and consistent repeat business. The company benefits from long-standing supplier relationships, efficient operating systems, and an experienced team that supports seamless daily operations. Well-established processes, a commitment to customer satisfaction, and a reputation for delivering dependable service have positioned the business as a trusted resource within its market. These strengths provide a solid operational foundation while allowing new ownership to step into a proven and successful business. This turnkey opportunity is well suited for an owner-operator, strategic buyer, or an existing company seeking to expand its presence within the industrial supply sector. With established operations, a loyal customer base, and a respected market position, the business offers an excellent platform for continued success and future growth under new ownership.

Service Businesses

Established auto recycling, towing, and vehicle storage company serving the Jacksonville market with multiple established revenue streams. The business operates from a 1-acre property with an approximately 5,000-square-foot warehouse, providing valuable space for vehicle storage, parts inventory, dismantling, and day-to-day operations. A key component of the operation is its auto recycling business. The company works with insurance companies to acquire wrecked and salvage vehicles, then dismantles and recycles those vehicles by recovering and selling usable parts. With established customer and commercial relationships, multiple revenue streams, specialized equipment, and an operating model that combines towing, storage, salvage vehicle acquisition, and parts recycling, the business offers a strong foundation for a new owner.

Building & Construction

Full-Service Plumbing, Remodeling & Restoration Company Jacksonville, FL Metro Area This full-service, commercial, home services company offers plumbing repair, remodeling, water damage restoration, new construction plumbing, and managed property services throughout Duval, Clay, Nassau, and St. Johns counties. The business maintains a strong recurring revenue base through relationships with property managers, warranty companies, and contractors, along with 24/7 emergency plumbing response. It is licensed and insured, with a strong reputation and high customer satisfaction, an established brand and digital presence, and a turnkey, systematized platform ready to scale under new ownership. Seller works part time (~15 hours per week) and has strong field manager and office manager on staff. No adjustment has been made to financials to account for one full time owner operator) He is retiring and will hold license, if needed, or staff member could attain plumbing license.

Retail

Profitable Sunoco gas station for sale in the downtown Jacksonville area, situated just 2 miles from Jacksonville Landing. This well-established neighborhood store, only half a mile from an I-95 exit, operates as an absentee-run business requiring minimal owner interaction. The station boasts various income streams from ATMs, gaming machines, fuel sales, and store sales. Additionally, there is space within the store for a small fast food concept, offering a unique business opportunity in the high-density residential area. Equipped with a T-Canopy and 2 new MPDs with EMV/chip card readers, the property has been recently renovated with fresh paint and LED lights.

Entertainment & Recreation

Step into a well-established VR gaming center with loyal customers and solid reputation. Detailed financials available post-NDA. Seller offers transition support for a seamless handover. Ideal opportunity for entering or expanding in the VR gaming industry with immediate operational capacity.

Health Care & Fitness

Acquire a turnkey swim school in a prime location with a strong community presence and well-established curriculum. This business offers year-round swim lessons for children of all ages and abilities, backed by highly trained instructors and positive online reviews.

Beauty & Personal Care

Unique opportunity to own an industry-leading wellness franchise, specializing in therapeutic massage, skin care services, and assisted stretching as part of a holistic wellness philosophy. With monthly memberships approaching 1000, your guests can enjoy monthly wellness sessions that make them feel special will gaining the benefits of the various services offered by a dedicated staff of 27 professionals including 13 licensed massage therapists and 4 aestheticians. The business also offers various specials, promotions, and a robust gift card program to drive new customer revenue. This beautiful 4200sq foot facility boosts 15 therapy rooms, a dedicated member lounge, and 2 couple’s rooms. As the buyer, you will gain the benefit of an original build out costs exceeding $500,000 plus a recent refresh cost of $90,000. Your business neighbors in a vibrant shopping area include Kohls, HomeGoods, Alta, Target, 5-Guys, Hobby Lobby, and Ross just to name a few. Current retiring owner of 13 years works approximately 20hrs per week and is willing to training the next owner of this incredible opportunity.

Automotive & Boat

Local hands-on boat repair and maintenance. Specializing in marine equipment sales in addition to engine service work on Mercury/MerCruisers and Yamaha. This shop is a licensed Merc/ MerCruiser repair, parts, supplies, and dealer. As a repair focused shop, they also supply parts used in servicing and outfitting all types of boats. Boat dealer / broker-type services Dealer and service provider in the marine industry. Routine service work Includes tune-ups, troubleshooting, and general upkeep typical for recreational boats (implied from their repair classification and service category). ________________________________________

Beauty & Personal Care

Explore this well-established Hair Salon and Barbershop with a solid 18-year presence in the community, boasting a loyal customer base and a prime location. The business features 12 stations, with 4 currently rented, yielding an annual rental income of $50,000. There is potential for additional revenue with 8 vacant stations, a massage room, and 2 manicure stations, which could collectively generate over $100,000 annually at full occupancy. This opportunity is ideal for an owner-operator, experienced stylist, or investor seeking a business with existing clientele and significant growth potential. The space is beautifully designed and ready for immediate operation. The current owner is retiring, and the property is leased.